Nano Silver Particles Modified with Pectin and Their Application in Biomedical

الملخص

        Nanomaterials are important for the formation of silver nano particles (AgNPs), silver and pectin (Ag @Pc) complex was synthesized by two methods, namely laser ablation (PLA)and electrochemical cell. A set of tests have been conducted for this overlay such as: X-ray diffraction (X-ray) Analysis, Fourier Transform-Infrared Spectroscopy(FTIR)Analysis, Ultra-Violet visible (UV-Viz) Analysis, Transmission Electron Microscopy (TEM) Analysis, Atomic Force Microscope(AFM)Analysis, Scanning Electron Microscope (SEM)Analysis. X-ray examinations showed that the material has a polycrystalline nanostructure, with an average size of 20 nm, the highest peak intensity was at the angle of θ=38 ֩, A spherical shape for particles was  a Face Center Cubic ( F.C.C) type with a dominant plane (111). However, there is little difference between the two methods with a level of crystallization. As for the FTIR examination, it was found that the ideal absorption at the beams wave numbers 1588 cm-1 and 3340 cm-1, which is identical to the elongation frequency for Assignments (-COO, -OH) as a stretching vibration, respectively for both methods. As for the UV-Vis examination, the absorption peak was at the wavelength of 437 nm. Further, for the TEM examination, the XRD examination was strengthened having the same Nano structured shapes as a spherical shape. As for the SEM images of Ag@Pc films revealed nanometer-sized objects AgNPs are globular and evenly distributed throughout the inspected region. A less regular shape of AgNPs may indicate nanoparticle agglomerates more. As for AFM the image of the AgNPs@pectin film is slightly blurred, suggesting the effect of viscosity for the biological part, the material was examined Antibacterial activity for four types of gram-negative and gram-positive bacteria, namely (Staphylococcus aureus, Salmonella tophi, Klebsiella pneumoniae and Escherichia coli (E-coli)) with inhibition zone which were approximability 23, 21, 18 and 19 mm, respectively with plasmolysis of the cell wall and separation of the cytoplasm. However, the nano products synthesized by laser method was less efficient than in the electrochemical method due to its concentration and less impurities. More, the medical part, application of the nano products on one type of cancers, which is   skin cancer. The concentration of the substance ranged from 70-160 μg / mole. Silver and pectin showed toxicity to cancer cells, so it inhibited them depending on the concentration and period of application. The time in this case was from 0 to 120 min, at which the effect of silver and silver @ pectin was suppression of related protein. Skin cancer, it took the same concentration, but it was noted that concentration and time were also taken into account, but it was noted that silver with pectin stopped the migration of cancer cells by 50%, while silver 60%, Silver by 30 %. As for skin cancer, for the same concentration of 70 – 160 μg/ mole for same period, it was observed that it reduces the protein concentration. Western blot method was used to find out the protein concentration.