Antimicrobial & Antioxidant Activity of a Novel Exopolysaccharide Production by Pseudomonas Savastanoi pv. Savastanoi Bacterium Isolated from Olive Knot

Abstract

    The present study aimed Isolation, characterization of  EPS from Pseudomonas savastanoi isolated from olive tree (olea europea) from different sites of  Mosul city, and selected the isolation to produce EPS, Seven isolates of P. savastanoi Pss1 , Pss2, Pss3 , Pss4 , Pss5 , Pss6, Pss7were selected as the best isolates producing EPS based on the appearance of colonies and their growth with a viscous mucous. The results of the secondary screening showed that the best isolate was P. savastanoi (Pss3), gave best yield of  EPS, produced on liquid King B Medium reached 3 g/l after three days of incubati on. The EPS composition Extracted from bacteria was characterized by using TLC and HPLC technique, the results showed that the main units that make up the product such as glucose, fructose and mannose, The results of the study revealed that the inhibitory that EPS towards the growth of some pathological microorganisms and the Antioxidant Activity by effect of EPS on DPPH scavenging ability.