Mutational Effect of Penicillium griseoroseum on Genetic Composition and Secondary Metabolites Production

Abstract

Spores of P. griseoroseum were gathered from the atmosphere, this facilitated the identification of the fungus by looking for a internal spacer (ITS) that was documented by NCBI, PQ721627. Following the application of ultraviolet light at a wavelength of 253nm to the conidial suspension of P. griseoroseum at different distances of 15 cm from the source (UV1=2, UV2=4, UV3=6) min., the extracts were evaluated by GC-MS with regard to their secondary metabolites. 1-O-p-Nitrobenzoyl-2,3,4,6-tetra-O-benzyl-.beta.-d-galactose, -Hexadecenoic acid, methyl ester, (Z)-, Triacontane, 11,20-didecyl-,d-Arabino-hexonic acid, 2-deoxy-3,4,5-tris-O-(trimethylsilyl)-, trimethylsilyl ester, bis, and Androsta-3,5-diene-3,17-diol, 3-(heptafluorobutanoate) 17-(pentafluoropropanoate) are among the new chemical compounds that were identified as a result of the mutations, there are also some compounds that have disappeared due to mutation such as 2- (N-Methyl-N-nitroamino)-4-[2,2,3,3,3-pentafluoro-1,1-bis(trifluoromethyl)propyl]-6 and Octatriacontane, 1,38-dibromo-  . To determine how much radiation altered the genetic composition of the fungus, six primer pairs were inserted into the DNA - OPC-08, OPL-07, OPA-10, OPA-18, and OPB-02 . There was a clear impact on the following primers : OPC-08, OPA-18, and OPB-02.