Production of single cell protein Using Mixed Microbial Inoculum Technology from Two Local Bacterial Isolates, Kytococcus sedentarius, and Pseudomonas oleovoran, Using Solid Medical Waste

الملخص

    This research was conducted to produce single-cell protein using a mixed inoculum of two local bacterial isolates, namely Pseudomonas oleovorans and Kytococcus sedentarius. The submerged culture technique was used in the production process using the double inoculum. After initial treatments, solid medical waste was used as the main carbon and energy source in the nutrient medium on which the two bacterial isolates were grown. Production optimization experiments were conducted to reach the best production of single-cell protein. The production optimization experiments aimed to reach the best conditions for optimal production. These experiments included studying the effect of each factor (PH, temperature, inoculum size, type and concentration of nitrogen source, and the effect of incubation duration). Optimizing the production conditions showed that the best single-cell protein production was at PH9, temperature 45°C, and inoculum size 2 ml of each of the two isolates/100 ml of the production medium. The best production was achieved using NaNo₃ at a medium concentration of 0.3 g/100 ml. Finally, the best production was obtained at an incubation period of 72 hours. After applying the optimum conditions, the best single-cell protein production was 1.712 g/100 ml of the production medium. The analysis and separation of the culture filtrate after single-cell protein extraction using gas chromatography (GC-MS) technique showed that this filtrate contains 15 active chemical compounds with vast and valuable applications. These compounds are Chloro-3,3-dimethyl-8--6phenyl-3,4-dihydro-1H-thiopy, Neopentane, Oxadiazole, 5-(4-tert-[1,2,4]butylphenoxymethyl)-3-(thiophen-2-yl)- and (4-Methoxyphenyl)-2-(4--2 trimethoxysilyloxy)propane and Methane, isocyanato And Silane, dimethyl(2-chloro-5-methylphenoxy)heptyloxy and Propanoic acid, anhydride and N, N'-(4-Methyl-m-phenylene)bis(p-toluenesulfonamide) and 1,3-Propanediamine and Vitamin E and Silane, dimethyl(2-chlorophenoxy)octyloxy and Vitamin E succinate and Vitamin E and Silane, dimethyl(2-chlorophenoxy)octyloxy And alpha.-Tocopherol- beta.-D-mannoside and Acetic acid, [(1,1-dimethylethyl)thio].