Morphological and Molecular Study of the Biofilm of Proteus Mirabilis Isolated from Urinary Tract Infections

Abstract

    Proteus mirabilis is one of the most common causes of urinary tract infections, especially in catheter injected patients. The aim of study was to investigate morphological and molecular study of the biofilm of Proteus mirabilis isolated from urinary tract infections. One hundred urine samples were collected from outpatients and inpatients at Al-Diwaniyah Teaching Hospital and the Maternity and Children's Hospital who were diagnosed with urinary tract infections. The results showed that molecular detection of the genes responsible for the bacteria under study was performed on all samples using PCR technique. After transferring the result of the multiplication in agarose gel, it was noted that 10 isolates (100%) of the isolates possessed the int1 genes under study. In general, the virulence factor (Biofilm) in P. mirabilis was investigated, as the results of the phenotypic detection showed the ability of the bacteria under study to produce biofilm in 10 isolates at a rate of 100%.