Investigating the Effects of 70 mW Laser Irradiation at 808 and 635 nm Wavelengths on Proliferation and Apoptotic Mechanisms in Colon Cancer Cells

Abstract

Recent research has shown that light-laser diode radiation is an effective therapy for wound healing, inflammation reduction, and cancer prevention. This study aimed to assess the biological response of human colon cancer cells subjected to a single laser irradiation dosage (2.5, 5, 7.5, and 10 J/cm2). After seeding human colon cancer cells onto laboratory plates, various dosages of an 808 and 635 nm laser were used to irradiate their exposure at 70 milliwatts,   cells exposed to radiation were then incubated for one day. Changes in cell proliferation have been examined using MTT (3-(4,5-Dimethylthiazole-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assays. The viability of cells was decreased in nm-irradiated cultures than in control cultures. The results showed that doses of 2.5, 5, 7.5, and 10 J/cm2 were sufficient to cause visible alterations in colon cancer cells.