Assessing the Efficacy of Antimicrobial Agents in Controlling Bacterial Contamination on Hides and Skins: Minimum Inhibitory Concentrations and Susceptibility Testing

Abstract

This study aimed to investigate the minimum inhibitory concentrations (MICs) of antimicrobial agents containing three different chemical materials: thiocyanomethyl thiobenzothiazole, potassium dimethyldithiocarbamate, and benzyloxymethanol. Each of these active ingredients was tested separately at different concentrations against reference bacteria, including Staphylococcus sp., Peribacillus sp., Bacillus sp., Corynebacterium sp., and Pseudomonas sp., those were previously obtained from leather industry. In addition, to assess the susceptibility of these isolates to eight different antibiotics: Cefotaxime, Amikacin, Spectinomycin, Tetracycline, Rifampicin, Cephalothin, Ampicillin, and Neomicin. The Kirby-Bauer disc diffusion susceptibility test method was employed on Mueller Hinton Agar. The concentrations ranged from 2500 µg/ml to 0.001 µg/ml for thiocyanomethyl thiobenzothiazole, 5000 µg/ml to 0.002 µg/ml for potassium dimethyldithiocarbamate, and 10000 µg/ml to 0.0047 µg/ml for benzyloxymethanol as the active ingredient. The results found that the growth of all tested isolates was inhibited after exposure to the concentrations of antimicrobial agents containing thiocyanomethyl thiobenzothiazole as an active ingredients started from 2500 µg/ml to 39.06 µg/ml, 5000 µg/ml to 156.25 µg/ml containing potassium dimethyldithiocarbamate, and 1000 µg/ml to 312 µg/ml containing benzyloxymethanol.