The Effect of Sage Tea Compounds on the Reproductive Organs of Mature Female Mice

Abstract

    In Iraq and other Arab countries, salvia officinalis, or sage, is widely available and it is used to make a well-liked herbal tea that women with reproductive disorders can sip on a daily basis. The goal of this study include examine the probability usage of this herb in female hormonal disturbance as estrogen replacement choice via determination of the primary characteristics of ovarian and uterine tissues in adult female mice administered sage extract through histological and physiological analysis. The experiment included 36 mature female mice divided into three main groups (12 female mice in each group) were given equal amount of distilled water to control group while single and double dose of saga tea (0.057 g/ kg B.W. and 0.114 g/ kg B.W. respectively to group 2 and 3) for duration of 28 days. Following a course of treatment, six female mice were chosen from each group anesthetized and have blood samples taken for a test on reproductive hormones. ovarian and uterine sections were prepared for histological examination according to the routine microscopically technique at 5µm thickness then stained in H&E stain and examined to detect the number and diameter of ovarian follicles also detect the variation in uterine layers thickness via ocular micrometer. Due to the reproductive hormone assays, groups with sage extract treatment showed an increased in serum levels of progesterone, FSH, LH, and E2. However, histological analysis of ovarian and uterine sections showed a significant increase (P>0.05) in the diameter of the ovary as well as in the thickness of the endometrium and myometrium. In addition, numbers and diameters of ovarian follicles (primary, secondary, Graff follicles, and the corpus luteum also indicated a significant increase (P>0.05(compared with control group. neither group’s 2 nor 3 treated sage extract showed dose-dependency.